Kristina Schaedlich

2 Questions 6 Answers 0 Followers

Questions related from Kristina Schaedlich

How do THP-1 cells normally look like after PMA treatment?

We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...

24 October 2017 4,770 1 View

Can anybody help with a problem with Western Blot from Adipocytes/adipose tissue?

I'm currently trying to do Western Blot analyses with adipocyte samples from SGBS culture (d14). The extraction buffer is RIPA and we are loading 20 µg of protein on the SDS gel (measured with BCA...

27 May 2014 3,300 5 View