Kristian E Swearingen

2 Questions 10 Answers 0 Followers

Questions related from Kristian E Swearingen

When using MCX to clean up peptides prior to mass spec, is desalting by C18 also necessary?

When preparing peptides for analycis by LC-MS, I have seen several protocols that use C18 for desalting after detergent removal by MCX. Other groups skip the C18 step. My sample has 0.1% SDS and...

04 April 2016 6,805 3 View

How can I elute a target from a biotinylated antibody without breaking the avidin-biotin bond?

I have an organelle of interest captured by an antibody. I would like to elute the organelle for downstream analysis by LC-MS. We expressed a cMYC tag on a surface protein, then captured the...

08 August 2013 926 18 View