Kristian E Swearingen

3 Questions 11 Answers 0 Followers

Questions related from Kristian E Swearingen

Do urea or SDS interfere with the capture of biotin by streptavidin?

Several sources indicate that, once bound, the streptavidin-biotin complex can withstand up to 2% SDS and 6M urea (at room temperature). However, I can't find any discussion on whether the...

07 October 2015 6,528 3 View

Does anyone have experience with elute biotinylated proteins without eluting avidin as well?

We are using Life Technologies MyOne Streptavidin T1 beads to pull biotinylated proteins out of a cellular lysate for downstream analysis by LC-MS. Following the manufacturer's protocol, I elute...

29 April 2014 9,782 43 View

How can I elute a target from a biotinylated antibody without breaking the avidin-biotin bond?

I have an organelle of interest captured by an antibody. I would like to elute the organelle for downstream analysis by LC-MS. We expressed a cMYC tag on a surface protein, then captured the...

21 August 2013 9,958 0 View