Krishna Raja Muthuraman

6 Questions 8 Answers 0 Followers

Questions related from Krishna Raja Muthuraman

Protocol for Biotin-Streptavidin conjugation?

Dear All, Protein A is expressed and biotinylated while Protein B is expressed with a fused monomeric streptavidin (mSA2). I would like to know the procedure for conjugating this protein A &...

17 October 2022 1,972 4 View

What Purification tag would be right for a 150 kDa recombinant protein?

I would like to know suggestions for a detection and purification tag for a 150 kDa recombinant protein that is to be expressed in insect cells. This protein would be used later for immunization...

05 August 2022 3,631 3 View

Does the His tag at C terminal of recombinant protein of ~20 kDa size affects in western blotting/ELISA?

Hi, My recombinant protein of size ~ 20kDa has a 6X His tag at its C-Terminal end. This recombinant proteins` purpose is to serve as a diagnostic antigen looking for antibody in the infected...

15 July 2017 2,427 7 View

Why DENV2 is the most commonly studied dengue virus serotype?

Most of the invitro studies were performed on dengue virus serotype 2. Why this serotype is given more importance than the others?

21 March 2017 4,517 5 View

Purification / separation of recombinant protein of interest from the digestion pool

Hi, I have cleaved the GST fused His-Tag from my expressed protein at the N terminal using Enterokinase enzyme. My protein of interest (17 to 20 kDa) is also having 6X His at its C terminal,...

17 November 2016 2,898 3 View

Which would be an apt kit for parasitic cDNA library construction, expression and immunoscreening by antibodies?

Hi, I am unable to find a suitable cDNA expression system for my parasite of interest - pork tapeworm, which can allow immunoscreening with antibodies. There are many publications citing, Lambda...

03 February 2016 1,787 0 View