Krishna Raja Muthuraman

5 Questions 8 Answers 0 Followers

Questions related from Krishna Raja Muthuraman

Protocol for Biotin-Streptavidin conjugation?

Dear All, Protein A is expressed and biotinylated while Protein B is expressed with a fused monomeric streptavidin (mSA2). I would like to know the procedure for conjugating this protein A &...

17 October 2022 1,967 4 View

What Purification tag would be right for a 150 kDa recombinant protein?

I would like to know suggestions for a detection and purification tag for a 150 kDa recombinant protein that is to be expressed in insect cells. This protein would be used later for immunization...

05 August 2022 3,621 3 View

Does the His tag at C terminal of recombinant protein of ~20 kDa size affects in western blotting/ELISA?

Hi, My recombinant protein of size ~ 20kDa has a 6X His tag at its C-Terminal end. This recombinant proteins` purpose is to serve as a diagnostic antigen looking for antibody in the infected...

15 July 2017 2,423 7 View

Purification / separation of recombinant protein of interest from the digestion pool

Hi, I have cleaved the GST fused His-Tag from my expressed protein at the N terminal using Enterokinase enzyme. My protein of interest (17 to 20 kDa) is also having 6X His at its C terminal,...

17 November 2016 2,892 3 View

Which would be an apt kit for parasitic cDNA library construction, expression and immunoscreening by antibodies?

Hi, I am unable to find a suitable cDNA expression system for my parasite of interest - pork tapeworm, which can allow immunoscreening with antibodies. There are many publications citing, Lambda...

03 February 2016 1,784 0 View