Krishan Gourav Behera

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Questions related from Krishan Gourav Behera

Should sucrose be added in the plant media (like MS or Hoagland) during any kind of abiotic stress treatment on the transgenic plants?

I have to do various types of phenotypic and physiological assays after the abiotic stress treatment on the transgenic tobacco plants. For that i have to grow them in plant media containing the...

12 April 2025 5,487 2 View

Can a gene be directly cloned in pET28a vector or i need to subclone it?

I have an insert size of 900 bp, trying to clone it in pET28a vector. But after every the transformation when i am doing colony pcr and digestion check i can only get the fragment of 500 bp. Why...

28 August 2023 2,451 3 View