Kostantine Koukoutselos

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Questions related from Kostantine Koukoutselos

How I can make sure a AM conjugated dyes is permeating the cell membrane?

I am trying to load calcium Green Tm AM inside the neurons. As a first step, I would like to ensure the dye permeates the cell membrane. I am using a 5x and 10x objective to quantify the...

10 October 2023 3,318 3 View

Could somebody explain how can we use TTX to distinguish between monosynaptic and polysynaptic circuits?

Two papers are using the method. One attached and the other cited here: "Single rodent mesohabenular axons release glutamate and GABA" Root et al 2014

18 February 2016 4,569 3 View