Kornel Milcarz

4 Questions 5 Answers 0 Followers

Questions related from Kornel Milcarz

Bacterial Two Hybrid - can it be used for fusion proteins with FP?

Hi, I encountered problem with my protein of interest. It's 20kDa protein which is expressed on very low lvls in M. smegmatis. During stress its lvl is elevated but not much. I wanted to...

21 February 2022 9,989 0 View

Can I use DreamTaq (Thermo) polymerase to a-tailing of PCR fragments?

I want to add dATP on the ends of to my PCR product (Phusion, Thermo). My question is if it's possible with use of DreamTaq polymerase (Thermo)? I find informations about Taq polymerases but can...

31 January 2022 4,882 8 View

Is it possible to fuse protein with two the same fluorescent proteins in order to get stronger fluorescence signal?

Hi, I'm working on protein which is expressed on very low lvls in mycobacterium smegmatis. I have fused this protein with mNeonGreen and i couldn't see any signal (probably due to it's low lvls)....

24 January 2022 2,440 6 View

How can I do my blot cleaner (high background noise)?

I got high background noise on my blots after detection. Can it be a problem due to protocol I'm using for western blot? After transfer I'm staining with Ponceaus S, i'm washing a bit with...

03 March 2020 1,542 1 View