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Hi everyone! Recently I was doing a lot screening of drug pairs in 5 osteosarcoma cell lines using this amazing website https://synergyfinder.fimm.fi. I am puzzled why a lot of combinations have...
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I will get a sample of bone marrow cells, containing appr. 1000 cells in 200 µl 1xPBS. For my downstream application, I would need to concentrate them down to 30-40 µl volume. Does anyone have...
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I seek for your advice on the strategy on 5' RACE if I have a sample with cDNA of the patient. As I understood from the literature search, I should start with the 5' priming of the cDNA. In this...
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