Konstantin Byrgazov

4 Questions 19 Answers 0 Followers

Questions related from Konstantin Byrgazov

Synergy scores: HSA and Bliss positive, but Loewe is negative. How to intepret?

Hi everyone! Recently I was doing a lot screening of drug pairs in 5 osteosarcoma cell lines using this amazing website https://synergyfinder.fimm.fi. I am puzzled why a lot of combinations have...

05 May 2018 4,999 1 View

Working with the ultra small amount of bone marrow cells - any thoughts?

I will get a sample of bone marrow cells, containing appr. 1000 cells in 200 µl 1xPBS. For my downstream application, I would need to concentrate them down to 30-40 µl volume. Does anyone have...

04 April 2014 2,718 3 View

Can anyone help with 5' RACE from the cDNA?

I seek for your advice on the strategy on 5' RACE if I have a sample with cDNA of the patient. As I understood from the literature search, I should start with the 5' priming of the cDNA. In this...

02 February 2014 4,336 6 View

Suggestions for a small (10-12 kDa) inert protein to be used in reporter system.

I am working on post-transcription regulation of mRNA encoding big protein (app. 110 kDa) in Escherichia coli. I expect that upon stress conditions the ribosome employs different start codon on...

10 October 2012 9,953 23 View