Konstantin A. Blagodatskikh

2 Questions 25 Answers 0 Followers

Questions related from Konstantin A. Blagodatskikh

Why two HEX/BHQ1 TaqMan probes color differs so much?

I got two TaqMan probes with the same fluorophore/quencher combination (HEX/BHQ1) but their 100mkM solution color (with the naked eye) totaly different. Does it so sequence dependent? Or it is...

09 September 2017 3,350 1 View

Is it possible to detect and quantify such a short oligonucleotide with a mirror structure by PCR?

TTCCTTGTGTTGGTT Now my only suggestion to use it as one of the primers on a synthetic DNA. Oligonucleotide is dissolved in blood samples.

07 July 2015 3,329 5 View