Komal Pekhale

4 Questions 7 Answers 0 Followers

Questions related from Komal Pekhale

No Clear band in DNA-EMSA.?

I have a protein whose function is to reduce transcription factors. To check whether my protein is active I have to do DNA-EMSA. I am using my purified protein to reduce TF from nuclear extract...

12 February 2016 4,120 5 View

How to express protein in picchia?

I have protein around 15kDa which i am unable to get in BL21 i tried many strains, but still no success. i also encounter with no induction though i got induced earlier. So can i get protein in...

10 December 2015 3,456 3 View

Any advice on late CT values of housekeeping genes?

i make cDNA of 1microgram RNA which is DNAse treated. while performing qPCR i am getting late CT values of the house keeping gene. i am unable to understand this because previously i use to get CT...

05 December 2015 6,495 3 View

What are the possible reasons for protein not expressed in Hela cells though mRNA levels are in good amount?

i am trying to understand the reasons for the Hela cell lines not efficiently expressing my protein though my control has been showing good amount of protein. To see whether my protein is present...

03 December 2015 3,647 6 View