Kokwai Fung

5 Questions 12 Answers 0 Followers

Questions related from Kokwai Fung

Spore coat proteins purification using FPLC with SDS-8 M urea sample buffer

My sample buffer contains 50 mM Tris-HCl, pH 6.8, 2% SDS, 8 M urea, 10% glycerol, 2% mercaptoethanol and 0.02% bromophenol blue for getting the spore coat proteins. I think I can get rid of...

10 October 2013 2,910 5 View

How to measure the protein concentration after SDS-8M urea treatment?

My sample buffer contains 50 mM Tris-HCl, pH 6.8, 2% SDS, 8 M urea, 10% glycerol, 2% mercaptoethanol and 0.02% bromophenol blue. This buffer is used to determine the spore coat proteins. After...

10 October 2013 8,459 22 View

Does anyone know how to recover the protein (bands) from polyacrylamide gel?

I have searched a few protocols on website. The one I got was to excise the wanted bands and put it into particular pore size microcentrifuge filter. After that, centrifuge it to elute the...

09 September 2013 613 28 View

How to get spores from B. cereus, B. licheniformis and B. stearothermophilus?

Although I know the best way for spore induction is to mix the nutrient medium with manganese ion, I couldn't get any spores from these microorganisms by using Malachite green and Safranin...

07 July 2013 7,611 8 View

Does anyone know how to extract spore coat proteins from B. cereus, B. licheniformis and G. stearothermophilus?

Generally, sonication and glass bead have been used for extracting the spore proteins of Bacillus sp. I used Hielscher UP400S sonicator to extract spore coat proteins for the species I stated...

07 July 2013 3,182 0 View