Koen van Wijk

4 Questions 5 Answers 0 Followers

Questions related from Koen van Wijk

Why do my MIN6 cells secrete loads of insulin when treated with low glucose?

I'm trying to do a glucose stimulated insulin secretion assay (GSIS) by adding glucose to MIN6 cells. But strangely the lower dose (3mM) glucose gives a higher insulin secretion than the higher...

08 June 2023 4,716 4 View

Is BioTASQ no longer available?

I found this paper discussing how to analyze G4 quadruplexes in cell culture using biotinylated Template-Assembled Synthetic G-quartet (TASQ). In the paper the authors state that they bought it...

08 July 2022 4,647 0 View

Few bands left after SDS-Page transfer, how to increase transfer efficiency?

Hi there, back again with another question about SDS-Page / Western blotting. I am planning to load 30 ug of protein onto a 5-20% gradient gel. Protein of interest (multiple) 260kd, 62kd, 40kd....

12 July 2019 504 1 View

G6Pase Mouseliver Western blot can anyone help me, possibly already resolved?

Update: A collegeau of mine and I sat down and talked about the experiment from front to back and then I realized i did something really stupid. I mixed up two protocols basicly because I was...

01 July 2019 5,441 3 View