Kit-yeng Sheng

6 Questions 3 Answers 0 Followers

Questions related from Kit-yeng Sheng

What would be the cause to DNA ladder merging together?

Hi, I am currently experiencing an issue with the separation of the DNA ladder. To improve, I have lowered the voltage from 70V to 55V and extended the run time, the 1x TAE buffer is new, and...

22 January 2025 6,212 4 View

Which plasmid extraction kit did you used to prepare plasmids for nucleofection used in human embryonic cell lines?

Hi, I am wondering if different plasmid extraction kits yields different quality of extracted plasmids, which subsequently affected some downstream analysis such as nucleofection in human...

29 August 2023 7,454 3 View

Using Zeocin selection for H9 human ESCs?

I am currently establishing a transgenic cell line (H9) using a plasmid containing Zeocin resistant gene. An antibiotic kill curve was performed ranging from 0.5 ug/mL to 1000 ug/mL. H9 WT cells...

14 August 2023 5,160 1 View

What is the difference between Geltrex and Matrigel used in primed hESCs culture?

I wonder what are the differences between Geltrex and Matrigel used in maintaining primed hESCs in feeder free condition. Is Geltrex is better than Matrigel in maintaining the pluripotency of...

14 August 2023 9,005 4 View

What is the most effective way to insert a 20kb large insert into human genome?

Hello. I intend to insert a 20kb gene cassette into human genome using the CRISPR-Cas9 approach coupled with homology arm directed repair. It is know that this approach is less efficient due to...

14 August 2023 8,425 1 View

Does anyone have experience with adult transgenic mouse brain dissociation followed by FACS?

I am trying to recover transgenic fluorescing expressing neurons from adult mouse brain. Papain dissociation system by Worthing was used in first few trials but yield no transgenic fluorescing...

18 December 2018 158 3 View