The bacterias are not getting successfully transformed in golden gate reaction,can I get some suggestions on how to get a successful cloning done?

I normally use 20 minutes of cold shock then 1 minute of heat shock at 42 degree celsius and 2 minutes of cold shock again and then I put in 1 ml of LB medium and keep in rotatory shaker for 1...

26 June 2022 2,968 0 View