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I am trying to normalize an iTRAQ data set. I first used "summed intensities" in MASCOT. A first look at the data showed that the reporter ion ratio of most of the "housekeeping proteins" (e.g....
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I have been trying to identify proteins in a complex sample using a 2D workflow with MALDI-TOF/TOF MS. During the RP-LC I fractionate the tryptic peptides through a 1 hour gradient into 150 MALDI...
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