Kimberly D Dyer

2 Questions 6 Answers 0 Followers

Questions related from Kimberly D Dyer

Can anyone direct me to a good protocol or give technical advice on cloning into extremely low copy plasmids/BAC?

I have been trying for months to clone a 4 kB fragment into a 6.4 kB BAC (I know it's very small for a BAC). I know my restriction enzymes used to prepare insert and vector are good (i.e drops...

05 May 2014 1,236 8 View

I have recently generated 5 Kb genome fragments from cDNA and would like to sequence these fragments. ABI BigDye isn't working. Any ideas?

I have sequenced with ABI BigDye many time in the past but now am getting junk from my direct sequencing reactions.

02 February 2013 3,831 19 View