Kimberly B Bjugstad

4 Questions 15 Answers 0 Followers

Questions related from Kimberly B Bjugstad

What is the "average" wet weight for dissected RAT brain areas: substantia nigra, striatum, septum, hippocampus, nucleus accumbens, or VTA?

I will be doing some discrete brain dissections and need to justifying my animal numbers. I want to pool my tissue samples to get a solution that is 700 mg/ml. Does anyone have information...

10 October 2013 9,604 10 View

Non-specific DAPI binding?

I de-cellularized brain sections and wanted to verify that there were no more cells. I used DAPI to do this since it is specific for nuclei. In my controls (picture A) there are obvious labeled...

09 September 2013 1,849 11 View

What is the best antibody to label all astrocytes?

We usually use anti-GFAP to label astrocytes, but this usually labels only reactive astrocytes. Has anyone used anything different? We are trying to see if a certain metabolic enzyme is...

03 March 2013 3,209 31 View

What's a good counterstain for DAB based IHC in brain tissues?

We've noticed that we can get really good DAB-nickle staining (black) for our marker of interest (in this case BrdU) but when we go to counterstain with cresyl violet the DAB appears to fade out...

01 January 2013 6,190 79 View