Kimberly D Dyer

3 Questions 4 Answers 0 Followers

Questions related from Kimberly D Dyer

Does anyone have experience sequencing a genome from an negative strand RNA virus (like RSV)?

I thought I could make CNDA and PCR portions of genome and sequence the PCR fragments but this isn't going as well as it should. Now I am trying to sub clone fragments and sequence - also not...

04 February 2013 6,821 13 View

I have recently generated 5 Kb genome fragments from cDNA and would like to sequence these fragments. ABI BigDye isn't working. Any ideas?

I have sequenced with ABI BigDye many time in the past but now am getting junk from my direct sequencing reactions.

01 February 2013 7,738 18 View

Does anyone have a protocol for generating dendritic cells from mouse bone marrow using GM-CSF?

I would like to make and characterize mouse bmDCs.

20 November 2012 9,500 11 View