Kim Yongjae

3 Questions 5 Answers 0 Followers

Questions related from Kim Yongjae

The problem in homologous recombination for gene knock out or integration into bacterial genome.....Why Many colonies with negative results ??

Hi I am under doctors course, Yong Jae Kim. I have recently severe problem in homologous recombination for gene integration and knock out into bacterial genome. I've already check no problem...

04 April 2018 993 2 View

How to integrate a specific gene which is already in genome ???

Hi . I want to integrate a gene which is already in the genome by homologous recombination. However, it is almost impossible to integrate because is is not a foreign gene. How can I...

05 May 2017 748 8 View

The problem on pcr with overhang primers. How to make pcr reaction with overhang primers clearly?

Hello. I have a problem on pcr with overhang primer The total length of primer is 90 bp and annealing region is 25bp GC content% of annealing site is 40~45% I want to extend the gene about 60 bp...

04 April 2017 315 1 View