Kim Yongjae

3 Questions 5 Answers 0 Followers

Questions related from Kim Yongjae

The problem in homologous recombination for gene knock out or integration into bacterial genome.....Why Many colonies with negative results ??

Hi I am under doctors course, Yong Jae Kim. I have recently severe problem in homologous recombination for gene integration and knock out into bacterial genome. I've already check no problem...

02 April 2018 8,750 2 View

Whay I can not find any success in gene integration by colony pcr???

Hi I have experiment with Klebsiella oxytoca for gene integration by homologous recombination. I have designed the linear DNA cassette with target gene and Cm. as selection marker. And The...

22 May 2017 6,227 5 View

The problem on pcr with overhang primers. How to make pcr reaction with overhang primers clearly?

Hello. I have a problem on pcr with overhang primer The total length of primer is 90 bp and annealing region is 25bp GC content% of annealing site is 40~45% I want to extend the gene about 60 bp...

21 April 2017 9,091 1 View