Kim Jung Hun

3 Questions 3 Answers 0 Followers

Questions related from Kim Jung Hun

Can anyone experienced cells dying after Mesenchymal stem cell isolation from mouse bone marrow?

I'm trying to isolate mouse bone marrow mesenchymal stem cells. My simple version protocol : 1. Whole bone marrow flushing with syringe and PBS. 2. large tissues removed with cell...

13 June 2022 6,646 3 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

07 December 2020 9,275 5 View

Could you help me with TRAP staining problem in tissue sample?

I made TRAP staining solution with Naphthol AS-MX(1mg/ml) and Fast red salt (1mg/ml) in DMF/sodium acetate buffer pH 5.0. but it only work in vitro osteoclast sample, not in vivo tissue sample...

14 July 2020 7,561 3 View