Killian O'Sullivan

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Questions related from Killian O'Sullivan

Targeted stripping of secondary antibody only from FFPE tissue: is it possible?

Hi, I routinely incubate FFPE samples with different primary antibodies to specific target proteins for IHC. The primarys are always rabbit IgG, usually monoclonal. We then use the same secondary...

17 January 2025 2,716 3 View

Discrepancy in the general consensus of measuring phycocyanin spectrophotometrically: OD615 or OD620?

I am testing various methods of extracting C-Phycocyanin from fresh Arthrospira platensis biomass. To quantify the phycocyanin concentration within my solvents after extraction, I use the...

30 December 2023 5,666 0 View