Kien Xuan Ngo

3 Questions 13 Answers 0 Followers

Questions related from Kien Xuan Ngo

Any good suggestions for ligating big plasmid (11 kb) with small insert (250 b)?

I have a problem ligating a small insert with larger vector. The ligation sites are XhoI and AgeI. After the double digestion I excised band for I and V and extracted DNA by glass milk, purified...

16 December 2013 7,276 4 View

How to deplete adenosine triphosphate (ATP) in solution containing a negatively charged surface protein?

I have tried to remove ATP by anion exchange dowex resin, however, I have also lost proteins due to their negatively charged surfaces. Can anyone suggest the safe methods to deplete ATP from my...

23 May 2013 7,100 10 View

Any suggestion for running electrophoresis with small ssDNA, RNA (12mers)?

I have a problem in the separation of small DNA by Agarose Gel Electrophoresis (though I optimized with different concentrations of gel). Separation was very poor and always very blurry, smear...

12 January 2013 8,110 18 View