Kian Haseli

2 Questions 3 Answers 0 Followers

Questions related from Kian Haseli

After direct coculture separation of MSCs and macrophages, how much MSC present is acceptable when doing bulk RNA seq?

I have tried to separate a direct coculture of MSCs (mesenchymal stromal cells) and macrophages to do bulk RNA seq on macrophages, as I want to find out how MSCs change the genetic expression on...

27 February 2023 5,248 3 View

How many dynabeads per cell is optimal to separate MSCs from macrophages?

I have directly cocultured macrophages and MSCs fro 48 hours and now I want to separate the coculture with CD90 antibody-coated magnetic Dynabeads to separate the cells by magnetic force. I have...

10 February 2023 154 4 View