How to prepare sample for low expressed secretary protein of Pichia pastoris pPCZaA, X-33 strain?

Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.

26 December 2023 9,001 0 View