2 Questions 3 Answers 0 Followers
Questions related from Khuram Shehzad
I have seeded keratinocytes in 6 well plate. 120 ul of RIPA buffer(with Pro. K) was used to break cells on ice. However, Bradford reagent (Biorad, diluted with water , 1:4) did not develop any...
18 July 2020 9,282 1 View
There is non specific elution of multiple proteins in which keep on reducing with increasing concentration of imidazol , along with target protein. I routinely use 500mM NaCl in samples and wash 1...
20 August 2014 8,772 2 View
