Khoa Tran

2 Questions 2 Answers 0 Followers

Questions related from Khoa Tran

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

14 December 2020 1,108 3 View

How to transduce Murine Erythroleukemia cells (MEL cells) using Lentivirus ?

Hi Everyone, I'm trying to tranduce MEL cells with lentivirus to knock out some genes with CRISPR Cas9 and select with puromycine. This is my first time working with this type of cells and...

01 April 2019 1,337 2 View