Why am I seeing nonspecific bands after switching to a rabbit anti-myc antibody for IB, and how can I fix this?

I transfected COS-7 cells with my plasmids of interest and performed co-IP using mouse anti-GFP for IP and mouse anti-myc for IB. I got positive results, confirming that my proteins of interest...

15 March 2025 9,263 3 View