How to make touch down PCR profile on thermocycler 71c to 61c 10 cycle and then 61c 15 cycle?

denature 94c for 2 minute then 10 cycle for 94c 30sec and annealing temp 71c to 61c then 15 cycle 61c annealing 72 c extension and final extension 72c for 4minute

24 March 2023 1,367 3 View