Khaled Essawi

3 Questions 5 Answers 0 Followers

Questions related from Khaled Essawi

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

24 February 2021 5,421 3 View

Promoters can be used to drive gRNA other than poly III (U6 and H1)?

I am trying to knockout gene at a specific location using CRISPR/Cas9 system but the issue is my spacer gRNA (20nt) contains 4Ts(TTTT) which signals as poly III terminator. If you have any idea...

07 December 2020 727 5 View

Is it critical to modify PAM site of my donor template?

I am trying to make correction for certain mutation using cas9+ corrective donor template. i have made modification in my gRNA sequence but not in PAM site and i am wondering if its really...

19 September 2019 9,620 0 View