After dialysis, my purified His-tagged fluorescent protein shows a high molecular weight band on SDS-PAGE?

I produced a fluorescent protein fused to a cell-penetrating peptide and purified it using anti-His magnetic beads. Elution was done with 50 mM Tris, 500 mM NaCl, and 250 mM imidazole at pH 7.4. I...

26 June 2025 1,986 10 View