Why cell wash off during plaque assay using A549?

Hi, I'm using A549 and PR8 H1N1 virus to do plaque assay and I'm facing a problem as my cells always wash off after I fix it using 4% formaldehyde. Currently, I'm using 2ug/ml TPCK, 0.6% agarose...

24 March 2023 822 0 View