Kevin Lee

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Questions related from Kevin Lee

Stripping membrane with low molecular weight proteins (<30kDa)? Western blot?

We currently use restore stripping buffer https://www.thermofisher.com/order/catalog/product/21059[1] which we usually incubate for 10-15 minutes to strip. For lower molecular weight proteins, we...

10 October 2015 5,665 4 View

I keep losing RNA doing Trizol RNA Extractions and Precipitations. Why don't I see pellets sometimes and other times I do?

Iv been having a lot of trouble recently losing RNA during my Trizol RNA Extractions and subsequent precipitations (with Sodium Acetate and Ethanol). In every group, there's always a few samples...

03 September 2015 322 5 View

Does anyone know about washing western blot membrane w/ TBST overnight?

so the primary antibody we're using is particularly dirty, and even with 5+ five minute washes in TBST, we still get a good amount of background. I know its not the blocking or secondary. I ...

06 June 2015 5,571 5 View