Kevin Dhondt

3 Questions 6 Answers 0 Followers

Questions related from Kevin Dhondt

Does TRE/TET-On mice develop immunity against a human transgene?

When a human transgene is expressed upon activation of TeT-On system, is there any immune rejection of the transgene ? I looked in a lot of paper using this system but this question was not...

12 December 2015 9,538 1 View

How would you quantify a specific p65 activation by qPCR?

We cannot use luciferase reporter gene in our experimental settings and we would like to quantify p65 activation by a mean that enable a standardization by the number of viable cells. We thought...

06 June 2015 8,648 3 View

Do you have an idea of a physical or chemical method suitable to destroy RNA (and DNA) from tissues before processing for paraffin embbeding ?

We need to get tissue free of RNA (and if possible DNA) of more than 500 pb long (for stupid but regulatory reasons). Tissues spend 2 weeks in PFA 4% also for regulatory reasons but it seems it...

01 January 2013 5,761 9 View