Kevin Loope

5 Questions 10 Answers 0 Followers

Questions related from Kevin Loope

Coverage requirements for de novo genome with Illumina + PacBio?

I'd like to sequence the genome of the gopher tortoise. The genomes of congeners are ~2.4Gb. I'm trying to decide how much coverage is necessary; we plan to run the sample on a portion of a...

13 January 2023 619 4 View

What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

11 February 2021 5,726 8 View

What is the correct formulation of this RIPA buffer for protein extraction/cell lysis?

A published method i'm trying to replicate calls for a RIPA buffer described as: "(1 M NaCl, 0.5 M EDTA, 1% Triton X100, 0.5 M Tris-Cl pH 7.4; with added 5 M DDT, 0.1 M PMSF, 5 M mercaptoethanol,...

30 May 2020 1,983 0 View

Why are microsatellite stutter peaks changing on repeated fragment analysis runs of same PCR product?

I've run a couple hundred samples using a microsatellite panel, and recently some of the runs show very exaggerated stutter peaks smaller than the true allele size. To troubleshoot the issue,...

02 April 2020 4,588 7 View

Has anyone used MClab as an alternative to ABI size standards for fragment analysis and microsatellite genotyping?

I recently stumbled across MCLab's alternative size standards, which appear to be very similar to ABI's LIZ and ROX 500 size standards. Has anyone used these for fragment analysis with ABI...

15 April 2016 3,788 3 View