Kevin Loope

3 Questions 9 Answers 0 Followers

Questions related from Kevin Loope

Coverage requirements for de novo genome with Illumina + PacBio?

I'd like to sequence the genome of the gopher tortoise. The genomes of congeners are ~2.4Gb. I'm trying to decide how much coverage is necessary; we plan to run the sample on a portion of a...

13 January 2023 604 4 View

What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

11 February 2021 5,691 8 View

Why are microsatellite stutter peaks changing on repeated fragment analysis runs of same PCR product?

I've run a couple hundred samples using a microsatellite panel, and recently some of the runs show very exaggerated stutter peaks smaller than the true allele size. To troubleshoot the issue,...

02 April 2020 4,572 7 View