Keunmin Ken Lee

3 Questions 10 Answers 0 Followers

Questions related from Keunmin Ken Lee

Can plasmid DNA electrophoresis show only linearized band and no supercoiled band?

Hello. I was looking back at some gel pictures of mine (because downstream work is not working...) and realized the electrophoresis for plasmid DNA is kind of odd. From what I know, when plasmid...

11 August 2021 2,464 7 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

15 February 2021 2,248 4 View

Why doesn't my gel electrophoresis and full sequencing results not agree with each other?

I have a plasmid that has been fully sequenced using primer walking method. The plasmid turns out to be 4000 bp long. (I don't have sequencing equipment so I sent my plasmid to a company that does...

06 October 2020 2,997 13 View