Kesav Kumar Bheemavarapu

3 Questions 4 Answers 0 Followers

Questions related from Kesav Kumar Bheemavarapu

How can I improve the yield of Plasmid DNA isolation?

Hi every one. I am facing problems in the yield of Plasmid DNA isolation. I need your suggestions: I am using high copy number plasmids like pTT5, pcDNA. I am using MDI Mega and Giga kits for...

13 May 2015 930 3 View

Is there any method to detect the mutation caused by Taq polymerase with less Proofreading capacity and low fidelity?

I have used a Taq Polymerase with less Proof reading and fidelity. I have a doubt that some mutation may be occurred during the PCR. One drawback is the polymerase doesn't have 3'-5' exonuclease...

12 May 2015 5,900 3 View

Is there any method to detect the mutation caused by Taq Polymerase with low proof reading capacity and low fidelity?

I have used a Taq Polymerase with less Proof reading and fidelity. I have a doubt that some mutation may be occurred during the PCR. One drawback is the polymerase doesn't have 3'-5' exonuclease...

12 May 2015 1,590 5 View