Designing a ddPCR probe to a single base mutation. Any advice on best location for mutation site to maximise discrimination between wt and mutant?

I need to design a ddPCR probe to a single base mutation. Can anyone advise on the best location for the mutation site (e.g. middle, or towards 5' end or 3' end of probe) to maximise...

24 October 2018 8,941 4 View