In your experience, what is the most effective osmolyte/chemical chaperone for improving the solubility of recombinant proteins in E. coli?

I see people commonly using sugars (e.g. sorbitol) or amino acids (e.g. arginine), as added to the growth medium at or before the time of induction, to help prevent the formation of inclusion bodies.

10 March 2015 6,999 6 View