Kenzi Saito

2 Questions 7 Answers 0 Followers

Questions related from Kenzi Saito

Is the efficiency of ara-flp neo deletion always 100 percent? Does single colonies after ara-flp induction have only neo-deleted plasmid?

Recently I performed arabinose-induced flp-mediated neo deletion in SW105 (DH10B derivative) and tried to confirm neo deletion by PCR. PCR primers bind to 5' and 3' flanking regions of upstream...

09 September 2012 7,527 1 View

Why does my PCR screening fail in BAC recombineering, even though antibiotics resistantce suggests successful BAC DNA transformation?

I'm working on BAC recombineering. I've done electroporation of BAC DNA into the SW105 cells and got antibiotics-resistant cells, indicating that my BAC DNA was successfully introduced into the...

08 August 2012 2,683 3 View