Is the efficiency of ara-flp neo deletion always 100 percent? Does single colonies after ara-flp induction have only neo-deleted plasmid?

Recently I performed arabinose-induced flp-mediated neo deletion in SW105 (DH10B derivative) and tried to confirm neo deletion by PCR. PCR primers bind to 5' and 3' flanking regions of upstream...

07 September 2012 7,419 1 View