6.9 kb and 5.5 kb bands not visible. What is causing this issue?

I isolated mouse genomic DNA and digested it with BglII at 37C overnight (5 ug DNA, 10x NEB Buffer 3.1, 20 U BglII, 0.3 ul RNAse cocktail, 0.3 ul BSA (20 mg/ml) in a total volume of 30 ul. I ran...

07 July 2016 1,500 2 View