How do I prepare 3% methyl cellulose solution (with E3) for Zebrafish larvae (5-6dpf) to be immobilized (Preferably with no opacity or "cloudiness")?

Previous attempts have led to cloudy/opaque solutions. Heat 150 mL of E3 media to 80 degrees Celcius. 15g of Methyl Cellulose is added and stirred for 20 minutes with the heat off with a magnetic...

02 December 2015 1,284 5 View