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I would like to avoid transferring PCR product from the PCR plate to a microplate.
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My boss wants me to run PCR with a fluorescent tag. He says we can use the Tecan SPARK machine that we have for this. I am confused however. Do I run the PCR per usual with the thermocycler then...
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If i have a gwas with lets say 100 individuals across 1000 SSR markers. WHat is the DOF? do i look at the individuals or the markers?
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