Kendall C. Case

2 Questions 10 Answers 0 Followers

Questions related from Kendall C. Case

What anti-HA antibody would you recommend using with yeast extracts?

I am looking for an HA-tag antibody to use with yeast. The HA-tagged proteins I am trying to assay via WB are endogenously tagged with 3xHA. I don't see very high levels of expression. Therefore,...

04 November 2021 4,623 7 View

Any tips for running low-concentration (< 6%) protein gel?

I'm trying my hand at Zn2+ Phos-tag gels. I have been working with 8% acrylamide, but my protein migrates through this very slowly. I attempted a 6% acrylamide and instead of waiting 5 hours for...

11 December 2019 3,276 7 View