Will dual index Illumina MiSeq run work if the Illumina 5' Adapter has been extended with GCT ? Sequencing company suggests this may cause issue?

I ordered 16S primers according to the earth microbiome website, however, the researchers added GCT to the end of the Forward primer Illumina 5' Adapter to affect the melting temperature of the...

14 August 2020 1,792 1 View