Kelly Hsieh

3 Questions 9 Answers 0 Followers

Questions related from Kelly Hsieh

I have problems with endocytosis studies. Why given the endocytosis inhibitors, protein uptake seemed to be increased? what went wrong?

I've been trying to do the endocytosis pathway study of cell penetrating peptide under different pH value. So far, I've tried several different inhibitors including chlorpromazine, MBCD and...

07 July 2016 7,053 8 View

SDS-PAGE: How to separate 28kDa and 30kDa?

I'm trying to use SDS-PAGE to separate my 28kDa and 30kDa proteins. I tried 4%-20% gradient gel (and ran for 1 hour more than I usually do to let my band come down to the bottom), but it didn't...

08 August 2015 2,810 8 View

How can I optimise protein purification (protein with his-tag) (using complete his tag purification resin by ROCHE)?

I'm purifying a protein with his-tag (N terminal 6xHis) by using cOmplete his tag purification resin (ROCHE). I've tried several conditions but I can't seem to find the best way to purify the...

08 August 2015 1,174 23 View