Kelly Velasco

3 Questions 3 Answers 0 Followers

Questions related from Kelly Velasco

How to prepare a alpha-ketoglutarate solution and store it?

Hi, I will do an enzymatic assay using a-ketoglutarate as substrate and I am wondering about the proper way to prepare and store this compound. As long as I could find out, it would be OK to...

10 September 2018 2,337 3 View

Which solvent should I use to dissolve Acetoacetyl CoA and how should I store the solution?

Hi! I am performing and enzymatic assay, where Acetoacetyl-CoA is the substrate. I have gotten the Acetoacetyl-CoA powder that SIGMA sells (5 mg). I think it would be easier to handle if I just...

13 March 2015 2,609 4 View

Which NADH do you recommend me to use for an enzymatic assay?

I want to use it as a co-factor of an enzymatic reaction, to evaluate the enzymatic performance of the enzyme and the kinetics of the reaction. I have read that it can be very tricky to work with...

02 February 2015 2,540 12 View