Kelly Hsieh

2 Questions 7 Answers 0 Followers

Questions related from Kelly Hsieh

How can I optimise protein purification (protein with his-tag) (using complete his tag purification resin by ROCHE)?

I'm purifying a protein with his-tag (N terminal 6xHis) by using cOmplete his tag purification resin (ROCHE). I've tried several conditions but I can't seem to find the best way to purify the...

31 August 2015 6,294 23 View

SDS-PAGE: How to separate 28kDa and 30kDa?

I'm trying to use SDS-PAGE to separate my 28kDa and 30kDa proteins. I tried 4%-20% gradient gel (and ran for 1 hour more than I usually do to let my band come down to the bottom), but it didn't...

19 August 2015 3,933 8 View