People who have successfully cultured E14 mESC (feeder free)- what FBS catalog and lot number did you use?

We've been attempting to thaw and expand E14 mESC in feeder free conditions. We bought new GMEM, LIF, but are still having issues with the culture. Our protocol is as follows: Plate dishes with 1%...

06 June 2016 8,312 3 View